元SeQuantの開発者による新しいHILICカラムです。新しい両性イオン型HILICカラムをお探しの方は特におすすめです。
Column:100 × 2.1 mm, 3.5 µm 100Å
Mobile phase:A) 100 mM ammonium formate, pH = 5.8 / acetonitrile = 30 /70
Flow rate:0.2 mL/min
Column temperature:Ambient
Injection volume:2 µL
LC–MS–MS system: Advance UHPLC with a CTC PAL autosampler and Bruker EVOQ™ Elite triple quadrupole system with electrospray ionization; multiple reaction monitoring mode (MRM) in ESI(+) and ESI(-) was used for selective and sensitive detection, see details in Table.
Column: 150 × 2.1 mm, 3.5-µm 100Å iHILIC-Fusion(+)
Gradient elution: A) acetonitrile; B) 10 mM ammonium formate, pH = 3.5; gradient elution from (95/5) A/B to (84/16) A/B in 8.5 min
Flow rate: 0.3 mL/min
Column temperature: 40 °C
Injection volume: 5 µL