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SunSec Diol 30, Diol 15

Infomation of end sales for SunSec series

SunSec series has been patronized for a long time.

Unfortunately the raw materials have become difficult to obtain, We will end sales as of the current stock.

Product Summary

Use target
Proteins, enzymes, peptides, saccharides, nucleic acids, etc.
Base particle
Porous silica having a surface coated with a hydrophilic compound comprising a hydroxyl group
Particle size
4 µm
Pore size
15 nm, 30 nm
Globular protein fraction range
3,000 - 200,000 (15 nm), 10,000 – 1,000,000 (30 nm)
Column material
Stainless steel
Column size
300 X 4.6 mm I.D.
Product Name Particle sie (µm) Globular protein fraction range
(protein)
Pore size (nm) Column size
I.D. x L (mm)
Number of theoretical plates Model number
SunSec Diol 15 4 1200000 15 4.6 x 300 32,000 or more DA4491
SunSec Diol 30 4 1000000 30 4.6 x 300 32,000 or more DB4491
Guard column
SunSec Diol-G
4 ----- ---- 4.6 x 30 ---- DG4431

Catalog   pdf

Features of SunSec

  • ★Employing two types of silica gel pore size of 30 nm and 15 nm
  • ★An inner diameter of 4.6 mm length 300 mm packed in a column of semi-micro size, sensitivity three times, solvent usage 1/3 compared to the conventional internal diameter 7.8 mm column
  • ★Achieve high durability of the column, sample injection number of times or more 500 times
  • ★4 µm high column packed with diol silica gel, guarantees 1.5 times the 32,000-stage conventional 5 µm column

Calibration curves with two proteins pore size

SunSec Diol 30 is pore size is 30 nm, has become exclusion limit molecular weight compared to the other 25 nm diol column is doubled, it is advantageous for the separation of the monoclonal antibody of the dimer, trimer.

...

Durability of the column

If you 4.2mL the eluent capacity at the time of the analysis of the one-time, up to 797 times the analysis of (3350 mL) to maintain more than 90% of the performance.

Column
SunSec Diol 30 4.6 x 300 mm
Injection volume
5 µL
Eluent
50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl
Flow rate
0.35 mL/min
Temperature
25 ºC
Detection
UV at 220 nm
Sample
a mixture of thyroglobulin, bovine serum albumin and ribonuclease A (0.03% each)
...

And batch-to-batch reproducibility

As shown in the chromatogram of the left diagram, reproducibility of high inter-batch was able to confirm.

Column
SunSec Diol 30 4.6 x 300 mm
Injection volume
5 µL
Eluent
50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl
Flow rate
0.35 mL/min
Temperature
25 ºC
Detection
UV at 220 nm
Sample
a mixture of thyroglobulin, bovine serum albumin and ribonuclease A (0.03% each)

BSA, the monoclonal antibody isolated example