Products
SunSec Diol 30, Diol 15
Infomation of end sales for SunSec series
SunSec series has been patronized for a long time.
Unfortunately the raw materials have become difficult to obtain, we ended sales.
Product Summary
- Use target
- Proteins, enzymes, peptides, saccharides, nucleic acids, etc.
- Base particle
- Porous silica having a surface coated with a hydrophilic compound comprising a hydroxyl group
- Particle size
- 4 µm
- Pore size
- 15 nm, 30 nm
- Globular protein fraction range
- 3,000 - 200,000 (15 nm), 10,000 – 1,000,000 (30 nm)
- Column material
- Stainless steel
- Column size
- 300 X 4.6 mm I.D.
| Product Name | Particle sie (µm) | Globular protein fraction range
(protein) |
Pore size (nm) | Column size
I.D. x L (mm) |
Number of theoretical plates | Model number |
|---|---|---|---|---|---|---|
| SunSec Diol 15 | 4 | 1200000 | 15 | 4.6 x 300 | 32,000 or more | DA4491 |
| SunSec Diol 30 | 4 | 1000000 | 30 | 4.6 x 300 | 32,000 or more | DB4491 |
| Guard column
SunSec Diol-G |
4 | ----- | ---- | 4.6 x 30 | ---- | DG4431 |
Features of SunSec
- ★Employing two types of silica gel pore size of 30 nm and 15 nm
- ★An inner diameter of 4.6 mm length 300 mm packed in a column of semi-micro size, sensitivity three times, solvent usage 1/3 compared to the conventional internal diameter 7.8 mm column
- ★Achieve high durability of the column, sample injection number of times or more 500 times
- ★4 µm high column packed with diol silica gel, guarantees 1.5 times the 32,000-stage conventional 5 µm column
Calibration curves with two proteins pore size
SunSec Diol 30 is pore size is 30 nm, has become exclusion limit molecular weight compared to the other 25 nm diol column is doubled, it is advantageous for the separation of the monoclonal antibody of the dimer, trimer.
Durability of the column
If you 4.2mL the eluent capacity at the time of the analysis of the one-time, up to 797 times the analysis of (3350 mL) to maintain more than 90% of the performance.
- Column
- SunSec Diol 30 4.6 x 300 mm
- Injection volume
- 5 µL
- Eluent
- 50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl
- Flow rate
- 0.35 mL/min
- Temperature
- 25 ºC
- Detection
- UV at 220 nm
- Sample
- a mixture of thyroglobulin, bovine serum albumin and ribonuclease A (0.03% each)
And batch-to-batch reproducibility
As shown in the chromatogram of the left diagram, reproducibility of high inter-batch was able to confirm.
- Column
- SunSec Diol 30 4.6 x 300 mm
- Injection volume
- 5 µL
- Eluent
- 50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl
- Flow rate
- 0.35 mL/min
- Temperature
- 25 ºC
- Detection
- UV at 220 nm
- Sample
- a mixture of thyroglobulin, bovine serum albumin and ribonuclease A (0.03% each)
BSA, the monoclonal antibody isolated example
